Coding

Part:BBa_K1401003:Design

Designed by: Kathleen Lewis   Group: iGEM14_BostonU   (2014-07-21)


MoClo Level 0 part of LmrA Repressible gene. TetR homolog


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 62
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 62
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 62
    Illegal AgeI site found at 80
    Illegal AgeI site found at 425
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Bacillus subtilis. The LmrA coding sequence came from Addgene plasmid 49364.

References